North Carolina

Johnny Randall, North Carolina Botanical Garden

This video reviews an experimental technique that refines optimal practice for planting the federally endangered harperella (Ptilimnium nodosum) along a river. After selecting a suitable site, obtaining permission, and propagating many plants, North Carolina Botanical Garden staff and volunteers planted 70 seedlings planted into replicated plots of terracell, coir, and natural cobble. Using the two stabilizers required different planting techniques.  Monitoring following planting day revealed that coir fabric supported the best plant growth even after many years.

Contributing Author(s): 
Date Recorded: 
Monday, August 26, 2019

Johnny Randall, North Carolina Botanic Garden (SePPCon 2016)

Johnny reviews the Center for Plant Conservation best practices related to the link between ex situ and in situ actions. Ex situ collections held as seeds or whole plants can help with research on germination or cultivation and reintroductions to the wild. He discusses clues that may trigger ex situ action, reviews the organizations that help guide the practice, and describes the link to the Global Strategy for Plant Conservation. He reviews patterns of where seed banking is practiced in the world and where it is needed and discusses the continuum of seed types that require different efforts for ex situ conservation. He briefly describes ethics and prioritization for collections, protocols for sampling genetic diversity, and techniques for processing orthodox seeds. Genetic concerns about ex situ collections include genetic drift, adaptation to cultivation, mutation accumulation and artificial selection.  He showcases the NCBG program and accomplishments with partners.

This work was presented at the Southeast Partners in Plant Conservation (SePPCon) 2016 Meeting. Learn more about SePPCon here.

Contributing Author(s): 
Date Recorded: 
Tuesday, November 1, 2016

Johnny Randall and Michael Kunz (North Carolina Botanical Garden, University of North Carolina at Chapel Hill), and Jamie Winshell, Corbin D. Jones and Gregory P. Copenhaver (Department of Biology and Integrative Program for Biological & Genome Sciences,University of North Carolina at Chapel Hill)

Venus’ flytrap (Dionaea muscipula) is the most widely recognized carnivorous plant, and endemic to only 100 km landward radius around Wilmington, North Carolina, USA. Although a few large populations occur on protected lands, the number of individuals is declining, entire populations are being extirpated, and a seemingly secure species is now vulnerable to local extinction and loss of wild genetic variation. We used Restriction site-Associated DNA sequencing (RAD-seq) to evaluate the genetic architecture of Venus flytrap populations across its entire range. In addition, we collected and banked over 25,000 seeds from 20 populations as a long-term conservation resource. Initial analysis of 160 RAD-seq derived markers indicate limited genetic variation within the first population sampled. Genetic variation was surprisingly heterogeneous across loci with some populations harboring appreciable variation and others harboring next to none. This initial analysis is ongoing for approximately 150 populations to provide a high-resolution assessment of the existing genetic variation, which will help guide future conservation efforts and understand species phylogeography.

Contributing Author(s): 
Date Recorded: 
Thursday, May 3, 2018